Invitrogen

Renalase Recombinant Rabbit Monoclonal Antibody (9A5T7)

Name: Renalase Recombinant Rabbit Monoclonal Antibody (9A5T7)
Brand: Invitrogen
SKU: MA5-38006

查看其他10个Renalase抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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Renalase Antibody (MA5-38006) in WB

Western blot analysis of Renalase in extracts of various cell lines. Samples were incubated with Renalase Monoclonal antibody (Product # MA5-38006) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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Immunogen image

Immunogen Image Renalase Recombinant Rabbit Monoclonal Antibody (9A5T7)

产品信息

MA5-38006

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

9A5T7

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Renalase (Q5VYX0)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.48 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2897924

产品详细信息

Positive Samples: Mouse liver, Mouse testis, Mouse kidney, Rat liver, Rat testis, Rat kidney

Immunogen sequence: MAQVLIVGAG MTGSLCAALL RRQTSGPLYL AVWDKAEDSG GRMTTACSPH NPQCTADLGA QYITCTPHYA KKHQRFYDEL LAYGVLRPLS SPIEGMVMKE

靶标信息

Renalase is a flavoprotein enzyme encoded by the RNLS gene, primarily expressed in the kidneys and heart where it plays a critical role in metabolizing catecholamines and catecholamine-like substances. It utilizes nicotinamide adenine dinucleotide (NADH) as a cofactor and operates through a superoxide-dependent mechanism, which is distinct from monoamine oxidase pathways. Renalase is considered a novel renal hormone that influences blood pressure and cardiac function by regulating circulating catecholamines. Its genetic variants have been associated with essential hypertension, highlighting its importance in cardiovascular health. In diabetic nephropathy, renalase exhibits protective effects, as demonstrated in genetically modified mouse models. These models have shown that renalase overexpression can mitigate renal damage, suggesting therapeutic potential. Renalase's expression is highest in the proximal tubules of the kidneys, which are densely innervated by sympathetic nerves, underscoring the connection between renalase and sympathetic nervous system activity. This enzyme's discovery has enriched our understanding of renal hormone regulation and its implications in diseases such as chronic kidney disease (CKD) and end-stage renal disease (ESRD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。