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Invitrogen
RhoB Polyclonal Antibody
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FIGURE: 1 / 2
RhoB Antibody (PA5-91839) in WB
Western blot analysis of RhoB in extracts of various cell lines. Samples were incubated with RhoB Polyclonal antibody (Product # PA5-91839) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-91839
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-190 of human RhoB (NP_0040311)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.68 mg/mL
Amount
68 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse brain; Cellular Location: Cell membrane, Cleavage furrow, Late endosome membrane, Lipid-anchor, Nucleus
Immunogen sequence: VPEVKHFCPN VPIILVANKK DLRSDEHVRT ELARMKQEPV RTDDGRAMAV RIQAYDYLEC SAKTKEGVRE VFETATRAAL QKRYGSQNGC I
Target Information
Rho-related GTP-binding protein (RhoB) mediates apoptosis in neoplastically transformed cells after DNA damage. While not essential for development, it does affect cell adhesion and growth factor signaling in transformed cells. RhoB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a tumor supressor - deletion of RhoB causes tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aplysia RAS-related homolog 6; aplysia ras-related homolog B; ARHB; h6; oncogene RHO H6; ras homolog B; ras homolog gene family, member AB; ras homolog gene family, member B; Rho; Rho cDNA clone 6; rho protein; Rho-related GTP-binding protein RhoB; rhoB; small GTP binding protein; unnamed protein product
Gene Aliases: AA017882; ARH6; ARHB; MST081; MSTP081; RHOB; RHOH6
UniProt ID: (Rat) P62747, (Mouse) P62746
Entrez Gene ID: (Human) 388, (Rat) 64373, (Mouse) 11852
mitotic cytokinesis
apoptotic process
actin filament organization
cell adhesion
signal transduction
small GTPase mediated signal transduction
Rho protein signal transduction
endosome to lysosome transport
positive regulation of endothelial cell migration
regulation of cell migration
positive regulation of cell migration
negative regulation of cell migration
positive regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell cycle
endothelial tube morphogenesis
cellular response to hydrogen peroxide
cellular response to ionizing radiation
regulation of modification of postsynaptic structure
negative regulation of establishment of endothelial barrier
angiogenesis
intracellular protein transport
obsolete transformed cell apoptotic process
cell differentiation
cytokinesis
transport
multicellular organism development
protein transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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