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Invitrogen
RhoGDI Polyclonal Antibody
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FIGURE: 1 / 5
RhoGDI Antibody (51-1000Z) in ICC/IF
Immunofluorescence analysis of RhoGDI Polyclonal Antibody was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RhoGDI Rabbit Polyclonal Antibody (Product # 51-1000Z) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488... View More
Product Details
51-1000Z
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 25 amino acid synthetic peptide derived from the N-terminus of the human Rho-GDI-alpha protein. This sequence is identical to the bovine sequence.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 47e; GDI-1; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; unnamed protein product
Gene Aliases: 5330430M07Rik; ARHGDIA; C87222; Gdi-1; Gdi1; GDIA1; HEL-S-47e; NPHS8; Rho-GDI; RhoDGI; RHOGDI; RHOGDI-1
UniProt ID: (Human) P52565, (Bovine) P19803, (Mouse) Q99PT1
Entrez Gene ID: (Human) 396, (Bovine) 338054, (Mouse) 192662
movement of cell or subcellular component
negative regulation of cell adhesion
regulation of Rho protein signal transduction
negative regulation of apoptotic process
positive regulation of GTPase activity
negative regulation of axonogenesis
positive regulation of axonogenesis
regulation of small GTPase mediated signal transduction
semaphorin-plexin signaling pathway
regulation of actin cytoskeleton reorganization
Rho protein signal transduction
regulation of cell shape
negative regulation of cell migration
regulation of protein localization
regulation of catalytic activity
cellular response to mechanical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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