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Invitrogen
Rhodopsin Monoclonal Antibody (1D4), APC
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Rhodopsin Antibody (MA5-45247) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Rhodopsin monoclonal antibody (Product # MA5-45247) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-45247
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1D4
抗原
Bovine Rhodopsin
偶联物
APC
APC
APC
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
1 µg/mL of MA5-45247 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
靶标信息
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: L opsin; long wavelength sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; red opsin; Rhodopsin; rod opsin; RP 4; unnamed protein product
基因别名: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
Entrez Gene ID: (Human) 6010, (Mouse) 212541
microtubule cytoskeleton organization
protein phosphorylation
G-protein coupled receptor signaling pathway
visual perception
phototransduction
phototransduction, visible light
response to light stimulus
detection of light stimulus
red, far-red light phototransduction
response to light intensity
gene expression
absorption of visible light
rhodopsin mediated signaling pathway
deactivation of rhodopsin mediated signaling
thermotaxis
photoreceptor cell maintenance
sensory perception of light stimulus
detection of temperature stimulus involved in thermoception
retina development in camera-type eye
cellular response to light stimulus
podosome assembly
transducin-mediated opsin signaling pathway
rod bipolar cell differentiation
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