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Agrisera
Rnr1/Ribonucleoside-diphosphate reductase large subunit Polyclonal Antibody
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Rnr1/Ribonucleoside-diphosphate reductase large subunit Antibody (AS21-4608) in WB
10 µL of total protein from 1 x 10^8 cells of Saccharomyces cerevisiae, extracted with lysis buffer ( 20 µm M Tris, p H 8,50 µm M Ammonium acetate, 2 µm M EDTA, plus protease and phosphatase inhibitor cocktails) containing 10% TCA, and re-suspended and denatured in 1 X LaemmLi buffer for 10 min, were separated on 10% SDS-PAGE and blotted on to nitrocellulose membrane (0.45 µm) for 1.5 h at constant 0.5 Ampe... View More
产品信息
AS21-4608
产品规格
种属反应
Yeast
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Two KLH-conjugated synthetic peptides derived from c-terminal of Saccharomyces cerevisiae Rnr1 protein.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Saccharomyces cerevisiae
靶标信息
Rnr1 is a nuclear-encoded, protein-coding gene whose product localizes predominantly to the cytoplasm and functions as the major large (alpha) subunit of the class I ribonucleotide reductase (RNR) complex, with regulated redistribution toward the nucleus under DNA replication stress. Rnr1 is a soluble enzyme subunit that forms an alpha2 catalytic core and associates with the small (beta) subunits to assemble the active alpha2beta2 RNR holoenzyme, in which the small subunit supplies the essential diferric-tyrosyl radical cofactor while Rnr1 provides the catalytic site and allosteric nucleotide-binding sites that control overall activity and substrate specificity. Biologically, Rnr1 catalyzes the rate-limiting reduction of ribonucleoside diphosphates to deoxyribonucleoside diphosphates, thereby sustaining balanced dNTP pools required for nuclear and mitochondrial DNA replication and for DNA repair; consistent with this central role, RNR1 expression is cell-cycle regulated and is also responsive to checkpoint signaling and metabolic cues (including iron availability) that tune dNTP production to replication demand and genotoxic stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATRNR1; CLS8; CRINKLY LEAVES 8; defective in pollen organelle DNA degradation 2; DPD2; R1; Ribonucleoside-diphosphate reductase large chain 1; ribonucleotide reductase 1; RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT 1; Ribonucleotide reductase R1 subunit 1
基因别名: CRT7; RIR1; RNR1; SDS12; YER070W
UniProt ID: (Baker's yeast) P21524
Entrez Gene ID: (Baker's yeast) 856801
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