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Agrisera
RpoC1/RNA polymerase beta subunit (chloroplast) Polyclonal Antibody
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RpoC1/RNA polymerase beta subunit (chloroplast) Antibody (AS15-2868) in WB
Samples: Left panel: Zea mays extracts: whole leaf (5 µg), chloroplast nucleoid (0.1 µg), thylakoid (5 µg), or stroma (5 µg) as indicated MW markers are marked 5 µg/well of total protein extracted from Arabidopsis thaliana and Zea mays in 100 m M Tris-HCL p H 7.5,10%glycerol, 5 m M EDTA and 2 MM EGTA buffer and denatured in 66 m M Tris-HCL p H 6.8,0.2% glycerol, 0.23% SDS, 600 m M BME at 50°C/10 min. Sample... View More
产品信息
AS15-2868
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
His-tagged, highly conserved fragment of Zea mays RpoC.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Zea mays
靶标信息
RNA polymerase beta1 is a large, essential DNA-directed RNA polymerase subunit found in the cytoplasm/nucleoid-associated transcription machinery of bacteria (exemplified by Escherichia coli rpoB), where it assembles with the other core subunits to form the catalytic enzyme responsible for transcription. The protein is a high-molecular-weight, multi-domain polypeptide (about 1.3k amino acids in E. coli) that contributes to the architecture of the active-center cleft and nucleic-acid binding channel, helping position the DNA template and nascent RNA during RNA chain initiation and elongation; its N-terminus is enriched in both acidic and basic residues, consistent with roles in macromolecular interactions. Functionally, the beta1 subunit is integral to the enzymatic activity of RNA polymerase, enabling accurate, processive synthesis of RNA from a DNA template using ribonucleoside triphosphates, thereby supporting global gene expression programs required for bacterial growth and stress responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: At4g35800; F4B14.70; NRPB1; RPB1
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