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Invitrogen
S-peptide Epitope Tag Monoclonal Antibody (6.2)
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产品信息
MA1-981
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans,
Tag
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
6.2
抗原
Synthetic Peptide: K(1) E T A A A K F E R Q H M D S(15) C
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody detects the S-peptide in human samples.
MA1-981 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot this antibody detects the S-peptide in K562 cells. In immunohistochemistry, and immunofluorescence procedures MA1-981 recognizes the S-peptide in transfected K562 cells.
The MA1-981 immunogen corresponds to the S-peptide conjugated through a C-terminal cysteine to keyhole limpet hemocyanin.
靶标信息
The S-Peptide epitope tag (S-Tag (TM)) can be used for numerous applications which include the analysis of localization, expression detection, and purification of polypeptides from mammalian cells. The small size of the tag (15 aa), the ability to purify tagged polypeptides without antibodies, and the very low expression of endogenous tissues in mammalian cells that react with S-Peptide make the S-Peptide antibody an attractive alternative to many existing epitope tags. Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
