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SAG12/Senescence-specific cysteine protease SAG12 Polyclonal Antibody
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SAG12/Senescence-specific cysteine protease SAG12 Antibody (AS14-2771) in WB
20 - 1.25 µg of soluble proteins from senescing or young leaves of oilseed rape (Brassica napus L. ) extracted with citrate sodium-phosphate buffer (100 m M, p H 6.8) and were separated on 12% SDS-PAGE and blotted 10 min to PVDF using semi-dry transfer (see Desclos et al. 2009). Blots were washed with TBS-T 3 times for 15 min at room temperature (RT) with agitation. Blot was incubated in the primary antibod... View More
产品信息
AS14-2771
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana SAG12 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare
靶标信息
SAG12 (senescence-associated gene 12) encodes a plant papain-like cysteine endopeptidase that is developmentally induced at late stages of tissue aging, with transcript accumulation largely restricted to senescing organs and repressed when developmental senescence is delayed by factors such as cytokinins, auxin, or sugar status. The gene produces a secretory-pathway protease precursor with an N-terminal signal peptide followed by an autoinhibitory prodomain typical of C1A (papain family) proteases, yielding a mature catalytic domain that carries the conserved cysteine peptidase active-site features; the protein has been experimentally localized to senescence-associated vacuoles, specialized acidic lytic compartments that arise in senescing leaf cells and are distinct from the central vacuole. Functionally, SAG12 is thought to contribute to macromolecule breakdown during senescence-supporting nutrient (notably nitrogen) remobilization for reproductive growth-by promoting proteolysis within these senescence-associated vacuoles, and its strong senescence-specific induction has made it a widely used molecular marker and promoter for senescence-targeted transgene expression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G45890; AtSAG12; CI0010; K15I22.9; K15I22_9
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