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Invitrogen
SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Polyclonal Antibody
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SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody (PA5-81795) in WB
Western Blot analysis using a SARS Coronavirus Spike Protein (subunit 1) antibody (Product # PA5-81795). Proteins were loaded on a reducing SDS-PAGE. Lane A: SARS-CoV Spike S1, 5 ng; Lane B: SARS-CoV2 Spike S1, 30 ng; Lane C: SARS-CoV2 Spike RBD, 30 ng. Separated proteins were transferred to a membrane and probed with PA5-81795 at 1:2000 dilution. The membrane was then probed with goat anti-rabbit IgG (H+L)... View More
产品信息
PA5-81795
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Human,
Plant,
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human SARS Coronavirus Spike S1 Subunit Protein (Met1-Arg667)
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Wet ice
RRID
产品详细信息
PA5-81795 has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Spike S1 and SARS-CoV-2 (2019-nCoV) Spike RBD.
PA5-81795 was prepared using the sequence encoding the S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667). This was expressed with a C-terminal polyhistidine tag.
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SARS Coronavirus Spike S1 Subunit Protein.
靶标信息
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
