NeoBiotechnologies

SATB2 (Colorectal Carcinoma Marker) Recombinant Mouse Monoclonal Antibody (rSATB2/6929)

Name: SATB2 (Colorectal Carcinoma Marker) Recombinant Mouse Monoclonal Antibody (rSATB2\/6929)
Brand: NeoBiotechnologies
SKU: 23314-MSM9-P1

查看其他47个SATB2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

SATB2 (Colorectal Carcinoma Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SATB2 (Colorectal Carcinoma Marker) Antibody (23314-MSM9-P1) in IHC (P)

IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using rSATB2/6929 at 2 µg/mL in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2nd Ab: HRP-polymer, 30 min. DAB, 5 min.

SATB2 (Colorectal Carcinoma Marker) Antibody in Immunoelectrophoresis (IE)

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Immunogen image

Immunogen Image SATB2 (Colorectal Carcinoma Marker) Recombinant Mouse Monoclonal Antibody (rSATB2/6929)

产品信息

23314-MSM9-P1

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫电泳 (IE)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

rSATB2/6929

抗原

Recombinant fragment (around aa200-300) of human SATB2 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody is stable for 24 months.

Positive Control: Human colon or cerebral cortex tissue. Cellular Localization: Nuclear

Specificity Comments: SATB2 is a DNA binding protein that specifically binds nuclear matrix attachment regions. It is involved in transcription regulation and chromatin remodeling. SATB2 expression in colorectal carcinomas (CRC) is correlated with good prognosis and in laryngeal squamous cell carcinoma it functions as a tumor suppressor wherein loss of expression is positively correlated with high tumor grade and recurrence. Moreover, SATB2, in combination with CK20, could identify almost all CRC s. Upper gastrointestinal (GI) carcinomas and pancreatic ductal carcinomas are usually negative for SATB2, and ovarian carcinomas, lung adeno carcinomas, and adeno carcinomas from other origin are rarely positive for SATB2. Additionally, SATB2 antibody can identify neuroendocrine neoplasms of colon and rectum because SATB2 is usually negative in neuroendocrine neoplasms of the GI tract, pancreas, and lung. More recently, it has been reported that SATB2 is a sensitive marker for tumors with osteoblastic differentiation.

靶标信息

DNA-binding protein SATB2 binds to DNA at the nuclear matrix- or scaffold associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. It also acts as a docking site for several chromatin remodeling enzymes by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. SATB2 is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes - probably by modulating BCL11B expression. It is a repressor of Ctip2 and a regulatory determinant of corticocortical connections in the developing cerebral cortex. Mutations in the gene are associate with cleft palate isolated (CPI).

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