Invitrogen

SDHA Monoclonal Antibody (2E3GC12FB2AE2)

Name: SDHA Monoclonal Antibody (2E3GC12FB2AE2)
Brand: Invitrogen
SKU: 459200

高级验证

60个已发表图片

106 篇参考文献

查看其他23个SDHA抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

SDHA Antibody in Immunocytochemistry (ICC/IF)

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SDHA Antibody (459200) in ICC/IF

Immunofluorescent analysis of SDHA in human embryonic lung-derived fibroblasts using an SDHA Monoclonal antibody (Product # 459200) at a concentration of 0.2 µg/mL. Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with an SDHA Monoclonal antibody (Product # 459200) at a concentration of 0.2 µg/mL, followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody at a concentration of 2 µg/mL.

SDHA Antibody in Immunohistochemistry (Frozen) (IHC (F))

SDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SDHA Antibody in Immunohistochemistry (IHC)

SDHA Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

SDHA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SH-SY5Y cells were transfected with SDHA siRNA and decrease in signal intensity was observed in Western Blot application using Anti-SDHA Monoclonal Antibody (2E3GC12FB2AE2) (Product # 459200). {KD}

Immunogen image

Immunogen Image SDHA Monoclonal Antibody (2E3GC12FB2AE2)

产品信息

459200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

查看83篇已发表文章 83篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:200

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

1 µg/mL

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看8篇已发表文章 8篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

C. elegans, Dog, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2E3GC12FB2AE2

抗原

Full length native protein (purified) corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline, pH 7.5

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10838019

产品详细信息

ICC application requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).

Positive control: Human heart mitochondria.

靶标信息

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Flavoprotein subunit of complex II; Fp; Malate dehydrogenase [quinone] flavoprotein subunit; mitochondrial succinate dehydrogenase complex subunit A; SDHA; succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex subunit A; succinate dehydrogenase complex subunit A, flavoprotein (Fp); succinate dehydrogenase complex, subunit A, flavoprotein (Fp); succinate dehydrogenase flavoprotein subunit A

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基因别名: 1500032O14Rik; 2310034D06Rik; 4921513A11; C81073; CMD1GG; FP; PGL5; SDH1; SDH2; SDHA; SDHF; SDHFP1