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Invitrogen
SEC31A Polyclonal Antibody
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SEC31A Antibody (PA5-52147) in ICC/IF
Immunofluorescence analysis of SEC31A was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SEC31A Polyclonal Antibody (Product # PA5-52147) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilu... View More
产品信息
PA5-52147
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 40% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: NWREALAAVL TYAKPDEFSA LCDLLGTRLE NEGDSLLQTQ ACLCYICAGN VEKLVACWTK AQDGSHPLSL QDLIEKVVIL RKAVQLTQAM DTSTVGVLLA AKMSQYANLL AAQGSIAAAL AFLPDNTNQP NIMQLR
Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.
靶标信息
The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ABP125; ABP130; Protein transport protein Sec31A; SEC31 homolog A, COPII coating complex component; SEC31-like protein 1; SEC31-related protein A; Web1-like protein; yeast Sec31p homolog
基因别名: ABP125; ABP130; HSPC275; HSPC334; KIAA0905; SEC31A; SEC31L1
UniProt ID: (Human) O94979
Entrez Gene ID: (Human) 22872
