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Invitrogen
SEMA3B Polyclonal Antibody
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SEMA3B Antibody (PA5-114387) in ICC/IF
Immunocytochemistry analysis of SEMA3B in A431 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
产品信息
PA5-114387
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human U2OS whole cell, human A549 whole cell, human U-87MG whole cell, rat thymus tissues, rat kidney tissue, mouse thymus tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue, mouse HEPA1-6 whole cel. IHC: human mammary cancer tissue, human colon cancer tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, rat brain tissue. ICC/IF: A431 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Seam V antibody; sema A; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; Sema V; semaphorin A; Semaphorin-3B; semaphorin-A; Semaphorin-V
基因别名: LUCA-1; SemA; SEMA3B; SEMA5; SEMAA; semaV
UniProt ID: (Human) Q13214
Entrez Gene ID: (Human) 7869, (Mouse) 20347, (Rat) 363142
neural crest cell migration
cell-cell signaling
axon guidance
positive regulation of cell migration
negative regulation of axon extension involved in axon guidance
negative chemotaxis
semaphorin-plexin signaling pathway
multicellular organism development
nervous system development
cell differentiation
