Invitrogen

SERCA1 ATPase Monoclonal Antibody (VE121G9)

Name: SERCA1 ATPase Monoclonal Antibody (VE121G9)
Brand: Invitrogen
SKU: MA3-912

高级验证

39个已发表图片

79 篇参考文献

查看其他16个SERCA1 ATPase抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

SERCA1 ATPase Antibody in Immunocytochemistry (ICC/IF)

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SERCA1 ATPase Antibody (MA3-912) in ICC/IF

Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (VE121G9) (Product # MA3-912) shows staining in A2058 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizin... View More

SERCA1 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SERCA1 ATPase Antibody in Western Blot (WB)

SERCA1 ATPase Antibody in Immunocytochemistry, Immunoprecipitation (ICC/IF, IP)

SERCA1 ATPase Antibody in Western Blot, Immunohistochemistry (WB, IHC)

SERCA1 ATPase Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Immunogen image

Immunogen Image SERCA1 ATPase Monoclonal Antibody (VE121G9)

产品信息

MA3-912

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看62篇已发表文章 62篇已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫组化（冰冻） (IHC (F))

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:500

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

查看2篇已发表文章 2篇已发表文章

亲和纯化 (AP)

查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Amphibian, Dog, Human, Mouse, Rabbit, Rat

已发表种属

Amphibian, C. elegans, Dog, Human, Mouse, Pig, Rabbit, Rat, Yeast

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

VE121G9

抗原

Purified rabbit skeletal muscle sarcoplasmic reticulum.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2061281

产品详细信息

MA3-912 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase in canine, amphibian, human, mouse, rat, and rabbit tissues.

MA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in mouse muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-912 results in strong labeling of the entire type II (fast) myofiber. MA3-912 has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate.

The MA3-912 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.

靶标信息

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: adult Ca2+ ATPase; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, ubiquitous; Ca2+ ATPase; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; neonatal Ca2+ ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; SERCA1; SR Ca(2+)-ATPase 1

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基因别名: ATP2A; ATP2A1; ATP2A3; SERCA1; SERCA1a