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Invitrogen
SERPINH1 Monoclonal Antibody (1C4-1A6), PerCP
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SERPINH1 Antibody (MA5-45303) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Hsp47 monoclonal antibody (Product # MA5-45303) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 antibody. (C) Composit... View More
产品信息
MA5-45303
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
1C4-1A6
抗原
Human HSP47, full length
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
1 µg/mL of MA5-45303 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects 47kDa.
靶标信息
SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors, and its expression is induced by heat shock. The SERPINH1 protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to SERPINH1 have been found in patients with rheumatoid arthritis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP 3; AsTP3; CBP 1; CBP 2; Cell proliferation-inducing gene 14 protein; collagen binding protein 1; Collagen-binding protein; Colligen; Colligin; Colligin 1; Colligin 2; colligin-1; colligin-2; HGNC 1547; Hsp 47; PIG 14; RA A47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
基因别名: AsTP3; CBP1; CBP2; gp46; HSP47; OI10; PIG14; PPROM; RA-A47; SERPINH1; SERPINH2
UniProt ID: (Human) P50454
Entrez Gene ID: (Human) 871
