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Agrisera
SGS3/Protein suppressor of gene silencing 3 Polyclonal Antibody
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SGS3/Protein suppressor of gene silencing 3 Antibody (AS15-3099) in WB
250 µg of grounded tissue from 2 weeks old Arabidopsis thaliana flowers from wt Col-0 and sgs 3 mutant was incubated with 1 m L lysis buffer (50 m M HCl p H=7.5,150 m M Na Cl, 10% glycerol, 5 m M Mg Cl 2,0,1% Nonidet P 40, Roche protease inhibitors, 1 m M TCEP) and denatured with LaemmLi buffer (2% SDS, 10% glycerol, 0.125 M Tris-HCl p H=6.8,1% DTT) at 85°C for 7 min. The samples were separated on 12% SDS-P... View More
产品信息
AS15-3099
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana SGS3 sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
SGS3, also called Suppressor of Gene Silencing 3, is a plant RNA binding protein essential for RNA directed DNA methylation and posttranscriptional gene silencing. It stabilizes double stranded RNA precursors by binding and protecting 3 prime overhangs, enabling RDR6 to synthesize secondary small interfering RNAs that amplify silencing signals. SGS3 localizes to cytoplasmic siRNA bodies and works with RDR6, DCL4, and AGO proteins to target viral RNAs, transposons, and aberrant endogenous transcripts. Its activity influences developmental regulation, antiviral defense, and stress responses by shaping small RNA populations and epigenetic marks. Loss of SGS3 impairs transgene and antiviral silencing, alters tasiRNA pathways, and leads to defects in leaf polarity, phase transitions, and resilience due to compromised small RNA biogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G23570; ATSGS3; MQM1.17; MQM1_17; SUPPRESSOR OF GENE SILENCING 3
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