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          • Primary Antibodies ›
          • SHH Antibodies

          Invitrogen

          SHH Polyclonal Antibody

          查看其他23个SHH抗体

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          Cite SHH Polyclonal Antibody

          Additional Information:
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          • 抗体检测数据 (1)
          SHH Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SHH Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 1

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          SHH Antibody (PA5-148850) in WB

          Western Blot analysis of SHH in human stomach and mouse stomach using SHH polyclonal antibody (Product # PA5-148850). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          SHH Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          SHH Polyclonal Antibody

          产品信息

          PA5-148850

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1 µg/mL
          -

          免疫组化(石蜡) (IHC (P))

          5-15 µg/mL
          -
          产品规格

          种属反应

          Mouse

          宿主/亚型

          Goat / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          E. coli-derived recombinant mouse Sonic Hedgehog, Cys25-Ser437 with a Lys122Arg substitution
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          浓度

          0.2 mg/mL

          规格

          100 µg

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 5% trehalose

          内含物

          no preservative

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3708064

          产品详细信息

          Reconstitute at 0.2 mg/mL in sterile PBS.

          靶标信息

          Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

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          引用该产品

          生物信息学

          蛋白别名: hemimelic extra toes; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh unprocessed N-terminal signaling and C-terminal autoprocfretinaessing domains; short digits; unnamed protein product

          查看更多 收起

          基因别名: 9530036O11Rik; Dsh; HHG-1; Hhg1; Hx; Hxl3; M100081; ShhNC

          查看更多 收起

          UniProt ID: (Mouse) Q62226

          查看更多 收起

          Entrez Gene ID: (Mouse) 20423

          查看更多 收起

          功能
          endopeptidase activity patched binding calcium ion binding protein binding glycosaminoglycan binding zinc ion binding morphogen activity hydrolase activity laminin-1 binding cholesterol-protein transferase activity
          参与通路
          negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels vasculogenesis osteoblast differentiation metanephros development branching involved in ureteric bud morphogenesis cell fate specification neural crest cell migration kidney development neural tube formation hair follicle development vasculature development heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation osteoblast development lymphoid progenitor cell differentiation regionalization developmental process involved in reproduction determination of left/right asymmetry in lateral mesoderm regulation of transcription, DNA-templated endocytosis signal transduction smoothened signaling pathway cell-cell signaling determination of left/right symmetry pattern specification process ectoderm development neuroblast proliferation axon guidance central nervous system development sensory organ development hindgut morphogenesis digestive tract mesoderm development heart development blood coagulation androgen metabolic process cell proliferation positive regulation of cell proliferation polarity specification of anterior/posterior axis anterior/posterior pattern specification dorsal/ventral pattern formation mesenchymal cell proliferation regulation of mesenchymal cell proliferation gene expression regulation of gene expression positive regulation of gene expression negative regulation of gene expression oligodendrocyte development striated muscle tissue development positive regulation of skeletal muscle cell proliferation myotube differentiation intein-mediated protein splicing protein autoprocessing spinal cord dorsal/ventral patterning ventral spinal cord interneuron specification spinal cord motor neuron differentiation lateral motor column neuron differentiation thalamus development forebrain regionalization dorsal/ventral neural tube patterning cell proliferation in external granule layer cerebellar granule cell precursor proliferation positive regulation of cerebellar granule cell precursor proliferation telencephalon regionalization zona limitans intrathalamica formation establishment of cell polarity regulation of proteolysis positive regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway respiratory tube development lung development embryonic limb morphogenesis negative regulation of cell migration male genitalia development prostate gland development thyroid gland development forebrain development midbrain development hindbrain development pancreas development hair follicle morphogenesis negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of neurotrophin production T cell differentiation in thymus regulation of immature T cell proliferation in thymus positive regulation of T cell differentiation in thymus positive regulation of immature T cell proliferation in thymus negative regulation of transcription elongation from RNA polymerase II promoter protein localization to nucleus embryonic forelimb morphogenesis embryonic hindlimb morphogenesis regulation of cell proliferation negative regulation of T cell proliferation negative regulation of protein catabolic process positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth odontogenesis regulation of odontogenesis embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process CD4-positive or CD8-positive, alpha-beta T cell lineage commitment tongue development tongue morphogenesis skin development positive thymic T cell selection negative thymic T cell selection intermediate filament organization cell fate commitment myoblast differentiation response to ethanol negative regulation of cell differentiation positive regulation of cell differentiation positive regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of photoreceptor cell differentiation positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation cell development thymus development digestive tract morphogenesis embryonic digestive tract morphogenesis embryonic organ development developmental growth embryonic morphogenesis embryonic foregut morphogenesis positive regulation of skeletal muscle tissue development organ formation anatomical structure formation involved in morphogenesis neuron fate commitment neuron fate specification response to axon injury embryonic skeletal system development oligodendrocyte differentiation positive regulation of oligodendrocyte differentiation smooth muscle tissue development branching morphogenesis of an epithelial tube male genitalia morphogenesis inner ear development formation of anatomical boundary stem cell development striated muscle cell differentiation positive regulation of striated muscle cell differentiation positive regulation of cell division Bergmann glial cell differentiation palate development limb development limb bud formation head development positive regulation of penile erection lung morphogenesis lung epithelium development trachea development trachea morphogenesis epithelial tube branching involved in lung morphogenesis branching involved in prostate gland morphogenesis branching involved in salivary gland morphogenesis bud outgrowth involved in lung branching right lung development left lung development lung lobe morphogenesis lung-associated mesenchyme development primary prostatic bud elongation prostate epithelial cord elongation mesenchymal-epithelial cell signaling salivary gland cavitation epithelial cell proliferation involved in salivary gland morphogenesis epithelial-mesenchymal cell signaling regulation of prostatic bud formation regulation of epithelial cell proliferation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development mesenchymal smoothened signaling pathway involved in prostate gland development artery development mesenchymal cell proliferation involved in lung development vasculogenesis involved in coronary vascular morphogenesis somite development positive regulation of sclerotome development fungiform papilla development fungiform papilla morphogenesis fungiform papilla formation positive regulation of oligodendrocyte progenitor proliferation cellular response to lithium ion dopaminergic neuron differentiation commissural neuron axon guidance alpha-tubulin acetylation metanephric mesenchymal cell proliferation involved in metanephros development metanephric collecting duct development negative regulation of canonical Wnt signaling pathway negative regulation of cholesterol efflux apoptotic signaling pathway regulation of protein localization to nucleus positive regulation of sprouting angiogenesis negative regulation of dopaminergic neuron differentiation tracheoesophageal septum formation negative regulation of ureter smooth muscle cell differentiation positive regulation of ureter smooth muscle cell differentiation negative regulation of kidney smooth muscle cell differentiation positive regulation of kidney smooth muscle cell differentiation positive regulation of mesenchymal cell proliferation involved in ureter development positive regulation of endothelial cell chemotaxis negative regulation of mesenchymal cell apoptotic process
          It has to be done as per old AB suggested Products section.

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