Invitrogen

SHMT2 Polyclonal Antibody

Name: SHMT2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-32228
Availability: InStock

高级验证

2个已发表图片

3 篇参考文献

查看其他15个SHMT2抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

SHMT2 Antibody in Immunocytochemistry (ICC/IF)

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SHMT2 Antibody (PA5-32228) in ICC/IF

Immunofluorescence analysis of Serine hydroxymethyltransferase, mitochondrial was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SHMT2 Polyclonal Antibody (Product # PA5-32228) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Seco... View More

SHMT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SHMT2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Serine hydroxymethyltransferase, mitochondrial was observed in Mouse and Rat Liver as compared to Mouse and Rat Skeletal muscle using Anti-SHMT2 Polyclonal Antibody (Product # PA5-32228) in Western Blot. {RE}

Immunogen image

Immunogen Image SHMT2 Polyclonal Antibody

产品信息

PA5-32228

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2549701

产品详细信息

Recommended positive controls: 293T, HeLa, A431, HepG2.

Predicted reactivity: Mouse (93%), Rat (91%), Xenopus laevis (81%), Rabbit (93%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Mammalian serine hydroxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible interconversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate in the cytoplasm (cSHMT, SHMT1) and mitochondria (mSHMT, SHMT2). cSHMT preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate, and inhibits SAM synthesis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。