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Invitrogen
SHP2 Polyclonal Antibody
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图: 1 / 5
SHP2 Antibody (PA5-95069) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of SHP2 in PC-3 cells using SHP2 Polyclonal Antibody (Product # PA5-95069). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95069
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human SHP2 (582-597aa RVYENVGLMQQQKSFR).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human CCRF-CEM whole cell, human Raji whole cell, human PC-3 whole cell, rat brain tissuerat C6 whole cellmouse brain tissuemouse 3T3-L1 whole cell. IHC: human lung tissue. ICC/IF: PC-3 cell. Flow: CACO-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: encodes catalytic domain; HCP; HGNC:9644; MGC14433; Noonan syndrome 1; null; OTTHUMP00000166108; protein tyrosine phosphatase SH-PTP2; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase SYP; PTP-1D; PTP1C; SH-PTP2; SH-PTP2 protein tyrosine phosphatase non-receptor type 11; SH-PTP2 protein tyrosine phosphatase, non-receptor type 11; SH2 domain-containing protein tyrosine phosphatase-2; SHP-2; Tyrosine-protein phosphatase non-receptor type 11
基因别名: 2700084A17Rik; AW536184; PTP1D; PTP2C; Ptpn11; SAP-2; SH-PTP2; SH-PTP3; SHP-2; Shp2; Syp
UniProt ID: (Mouse) P35235, (Rat) P41499
Entrez Gene ID: (Mouse) 19247, (Rat) 25622
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
non-membrane spanning protein tyrosine phosphatase activity
SH3/SH2 adaptor activity
insulin receptor binding
protein binding
hydrolase activity
phosphatase activity
protein domain specific binding
receptor tyrosine kinase binding
D1 dopamine receptor binding
phospholipase binding
insulin receptor substrate binding
peptide hormone receptor binding
protein phosphatase
DNA damage checkpoint
activation of MAPK activity
protein dephosphorylation
lipid metabolic process
triglyceride metabolic process
epidermal growth factor receptor signaling pathway
integrin-mediated signaling pathway
axonogenesis
brain development
heart development
hormone-mediated signaling pathway
positive regulation of signal transduction
dephosphorylation
platelet formation
microvillus organization
abortive mitotic cell cycle
regulation of cell adhesion mediated by integrin
negative regulation of cell adhesion mediated by integrin
multicellular organism growth
organ growth
peptidyl-tyrosine dephosphorylation
megakaryocyte development
atrioventricular canal development
ERBB signaling pathway
regulation of multicellular organism growth
hormone metabolic process
glucose homeostasis
positive regulation of mitotic cell cycle
negative regulation of insulin secretion
regulation of protein export from nucleus
positive regulation of hormone secretion
negative regulation of hormone secretion
platelet-derived growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
ephrin receptor signaling pathway
multicellular organismal reproductive process
genitalia development
inner ear development
homeostasis of number of cells within a tissue
negative regulation of cortisol secretion
negative regulation of growth hormone secretion
face morphogenesis
intestinal epithelial cell migration
positive regulation of ERK1 and ERK2 cascade
positive regulation of glucose import in response to insulin stimulus
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