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Invitrogen
SIAH1/SIAH2 Polyclonal Antibody
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SIAH1/SIAH2 Antibody (PA5-36274) in IHC (P)
Immunohistochemistry analysis of SIAH1/SIAH2 in paraffin-embedded human stomach carcinoma tissue (membrane and cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with SIAH1/SIAH2 polyclonal antibody (Product # PA5-36274) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
产品信息
PA5-36274
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to amino acids 178-229 of human Siah-1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody detects endogenous protein at a molecular weight of 40 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; FLJ08065; hSIAH1; mSiah2; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 1A; seven in absentia 2; Seven in absentia homolog 1; Seven in absentia homolog 2; Siah-1; Siah-1a; Siah-2
基因别名: AA415433; hSiah2; HUMSIAH; SIAH; SIAH1; SIAH1A; SIAH2; Sinh2
UniProt ID: (Human) Q8IUQ4, (Human) O43255, (Rat) Q920M9, (Mouse) Q06986, (Rat) Q8R4T2
Entrez Gene ID: (Human) 6477, (Human) 6478, (Rat) 140941, (Mouse) 20439, (Rat) 140593
ubiquitin-protein transferase activity
protein binding
protein C-terminus binding
zinc ion binding
ligase activity
identical protein binding
ubiquitin protein ligase activity
transcription corepressor activity
ubiquitin conjugating enzyme binding
protein homodimerization activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
ubiquitin-dependent protein catabolic process
apoptotic process
cell cycle
spermatogenesis
nervous system development
axon guidance
anatomical structure morphogenesis
protein catabolic process
protein destabilization
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cellular protein metabolic process
neuron apoptotic process
positive regulation of intrinsic apoptotic signaling pathway
small GTPase mediated signal transduction
regulation of protein ubiquitination
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
cellular protein catabolic process
negative regulation of canonical Wnt signaling pathway
negative regulation of nucleic acid-templated transcription
negative regulation of extrinsic apoptotic signaling pathway
male meiosis I
multicellular organism development
post-embryonic development
cell differentiation
regulation of multicellular organism growth
protein ubiquitination
