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Invitrogen
SIN3A Polyclonal Antibody
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SIN3A Antibody (PA1-870) in ICC/IF
Immunofluorescent analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sin3A polyclonal antibody (Product # PA1-870) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated ... View More
产品信息
PA1-870
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-870 detects mSin3A from human and mouse tissues and cells.
PA1-870 has been successfully used in Western blot, ICC/IF ChIP, IHC-P and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing mSin3A from T47D cell extract.
PA1-870 immunogen is a synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.
靶标信息
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; SIN3A; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3 yeast homolog A; transcriptional regulator, SIN3A
基因别名: AW553200; Kiaa4126; mKIAA4126; mSin3A; Sin3; SIN3A
UniProt ID: (Human) Q96ST3, (Mouse) Q60520
Entrez Gene ID: (Human) 25942, (Mouse) 20466
transcription regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
RNA polymerase II repressing transcription factor binding
RNA polymerase II transcription corepressor activity
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA binding
histone deacetylase activity
protein binding
protein complex binding
protein deacetylase activity
DNA binding
transcription corepressor activity
transcription factor binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
activation of innate immune response
positive regulation of defense response to virus by host
hematopoietic progenitor cell differentiation
DNA replication
transcription, DNA-templated
protein deacetylation
aging
regulation of hormone levels
positive regulation of G2/M transition of mitotic cell cycle
histone deacetylation
positive regulation of chromatin silencing
cellular protein localization
negative regulation of circadian rhythm
negative regulation of apoptotic process
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
cellular lipid metabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
response to methylglyoxal
cellular response to glucose stimulus
negative regulation of protein localization to nucleus
negative regulation of histone H3-K27 acetylation
cellular response to dopamine
negative regulation of transcription regulatory region DNA binding
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to organonitrogen compound
