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SIRT2 Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

SIRT2 Antibody in Immunocytochemistry (ICC/IF)

SIRT2 Antibody in Immunocytochemistry (ICC/IF)

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SIRT2 Antibody (600-401-EL6) in ICC/IF

Immunofluorescence Microscopy of SIRT2 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: SIRT2 antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: SIRT2 as a red fluorescent signal.

SIRT2 Antibody in Immunocytochemistry (ICC/IF)

SIRT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SIRT2 Antibody in Western Blot (WB)

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SIRT2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence Microscopy of SIRT2 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: SIRT2 antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: SIRT2 as a red fluorescent signal.

SIRT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of SIRT2 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SIRT2 antibody at 2.5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SIRT2 is nuclear and occasionally cytoplasmic. Staining: SIRT2 as a precipitated red signal.

SIRT2 Antibody in Western Blot (WB) — Western Blot of SIRT2 antibody. Lane A: Mouse brain lysate at 1 µg/mL. Lane B: Mouse brain lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 43.1 kDa, ~51 kDa for SIRT2.

Immunogen image

SIRT2 Polyclonal Antibody

Immunogen Image SIRT2 Polyclonal Antibody

产品信息

600-401-EL6

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

-

免疫组化（石蜡） (IHC (P))

2.5 µg/mL

-

免疫细胞化学 (ICC/IF)

5 µg/mL

-

酶联免疫吸附实验 (ELISA)

1:10,000-1:20,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-SIRT2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide near the C-terminus of the human SIRT2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 5E5 antigen; mSIR2L2; NAD-dependent deacetylase sirtuin-2; NAD-dependent protein deacetylase sirtuin-2; NAD-dependent protein defatty-acylase sirtuin-2; Regulatory protein SIR2 homolog 2; silent information regulator 2; silent mating type information regulation 2, (S.cerevisiae, homolog)-like; sirtuin 2; SIR2-like protein 2; sir2-related protein type 2; sirtuin type 2; sirtuin-2

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基因别名: 5730427M03Rik; SIR2; SIR2L; SIR2L2; SIRT2

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UniProt ID: (Human) Q8IXJ6, (Mouse) Q8VDQ8

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Entrez Gene ID: (Human) 22933, (Mouse) 64383, (Rat) 361532

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chromatin binding NAD+ ADP-ribosyltransferase activity histone deacetylase activity protein binding transcription factor binding zinc ion binding NAD-dependent histone deacetylase activity protein deacetylase activity NAD-dependent protein deacetylase activity histone acetyltransferase binding histone deacetylase binding tubulin deacetylase activity ubiquitin binding NAD-dependent histone deacetylase activity (H4-K16 specific) beta-tubulin binding NAD+ binding protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity metal ion binding N-acetylgalactosamine-6-phosphate deacetylase activity NAD binding diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity molecular_function

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated protein deacetylation autophagy cell cycle mitotic nuclear division nervous system development negative regulation of cell proliferation negative regulation of autophagy negative regulation of peptidyl-threonine phosphorylation phosphatidylinositol 3-kinase signaling histone deacetylation myelination in peripheral nervous system cell differentiation regulation of myelination positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to oxidative stress peptidyl-lysine deacetylation cellular response to hepatocyte growth factor stimulus negative regulation of protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of DNA binding protein kinase B signaling cellular lipid catabolic process regulation of fat cell differentiation negative regulation of fat cell differentiation positive regulation of meiotic nuclear division negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter hepatocyte growth factor receptor signaling pathway negative regulation of oligodendrocyte differentiation cell division meiotic cell cycle regulation of cell cycle positive regulation of cell division positive regulation of attachment of spindle microtubules to kinetochore negative regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to caloric restriction negative regulation of oligodendrocyte progenitor proliferation histone H3 deacetylation histone H4 deacetylation cellular response to molecule of bacterial origin cellular response to hypoxia cellular response to epinephrine stimulus tubulin deacetylation positive regulation of execution phase of apoptosis positive regulation of oocyte maturation negative regulation of NLRP3 inflammasome complex assembly negative regulation of defense response to bacterium ripoptosome assembly involved in necroptotic process negative regulation of reactive oxygen species metabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxia central nervous system development substantia nigra development chromatin silencing at rDNA chromatin silencing chromatin silencing at telomere protein ADP-ribosylation regulation of exit from mitosis gene silencing regulation of phosphorylation innate immune response response to redox state