Invitrogen

SLIT2 Polyclonal Antibody

查看其他12个SLIT2抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (3)

SLIT2 Antibody in Immunocytochemistry (ICC/IF)

SLIT2 Antibody in Immunocytochemistry (ICC/IF)

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SLIT2 Antibody (PA5-115924) in ICC/IF

Immunocytochemistry analysis of SLIT2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115924). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

SLIT2 Antibody in Immunocytochemistry (ICC/IF)

SLIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SLIT2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SLIT2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SLIT2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115924). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

SLIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SLIT2 in human liver cancer and para-carcinoma tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SLIT2 polyclonal antibody (Product # PA5-115924) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

SLIT2 Antibody in Western Blot (WB) — Western blot analysis of SLIT2 in COLO205 whole lysates. Samples were incubated with polyclonal antibody (Product # PA5-115924).

Immunogen image

SLIT2 Polyclonal Antibody

Immunogen Image SLIT2 Polyclonal Antibody

产品信息

PA5-115924

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SLIT2(Accession O94813), corresponding to amino acid residues N465-T515.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total SLIT2.

靶标信息

SLIT2 are ligands for Robo receptors and play a vital role for axonal migration/navigation during neural development. A number of cleavage products through alternate splicing are reported in the literature for SLIT2 proteins. The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, and adult spinal cord.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Cite this product

生物信息学

蛋白别名: leucine-rich repeat EGF-like repeat secreted protein; secreted ligand for robo receptors; slit-2; unnamed protein product

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基因别名: SLIL3; Slit-2

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UniProt ID: (Human) O94813

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Entrez Gene ID: (Human) 9353, (Rat) 360272

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GTPase inhibitor activity calcium ion binding protein binding heparin binding identical protein binding protein homodimerization activity laminin-1 binding proteoglycan binding chemorepellent activity Roundabout binding follicle-stimulating hormone receptor activity heparan sulfate proteoglycan binding

metanephros development ureteric bud development in utero embryonic development kidney development negative regulation of protein phosphorylation cell migration involved in sprouting angiogenesis negative regulation of leukocyte chemotaxis aortic valve morphogenesis pulmonary valve morphogenesis G-protein coupled receptor signaling pathway axonogenesis axon guidance motor neuron axon guidance negative regulation of cell proliferation negative regulation of lamellipodium assembly negative regulation of endothelial cell migration negative regulation of gene expression negative regulation of smooth muscle cell migration spinal cord development olfactory bulb development chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration chemorepulsion involved in postnatal olfactory bulb interneuron migration corticospinal neuron axon guidance through spinal cord telencephalon cell migration negative regulation of cell growth negative regulation of cell migration negative regulation of axon extension negative regulation of actin filament polymerization retinal ganglion cell axon guidance response to nutrient levels cellular response to hormone stimulus dorsal/ventral axon guidance Roundabout signaling pathway cellular response to platelet-derived growth factor stimulus follicle-stimulating hormone signaling pathway positive regulation of apoptotic process negative regulation of vascular permeability branching morphogenesis of an epithelial tube neuron projection morphogenesis axon extension involved in axon guidance negative chemotaxis induction of negative chemotaxis negative regulation of small GTPase mediated signal transduction response to cortisol positive regulation of synapse assembly synapse maturation ventricular septum morphogenesis mammary gland duct morphogenesis mammary duct terminal end bud growth apoptotic process involved in luteolysis negative regulation of chemokine-mediated signaling pathway cellular response to hypoxia cellular response to heparin negative regulation of smooth muscle cell chemotaxis negative regulation of mononuclear cell migration negative regulation of neutrophil chemotaxis negative regulation of monocyte chemotaxis negative regulation of retinal ganglion cell axon guidance negative regulation of cellular response to growth factor stimulus cell-cell adhesion neuron projection extension negative regulation of neuron projection development regulation of cellular component size regulation of neurogenesis regulation of axonogenesis positive regulation of axonogenesis epithelial tube morphogenesis circulatory system development