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Invitrogen
SLIT2 Polyclonal Antibody
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FIGURE: 1 / 9
SLIT2 Antibody (PA5-31133) in IHC (P)
Immunohistochemistry (Paraffin) analysis of SLIT2 was performed in paraffin-embedded mouse fetal brain tissue using Green: NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:200. Blue: Nuclear staining with Hoechst 33342. The signal was developed using a goat anti-rabbit IgG antibody.
Product Details
PA5-31133
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SLIT2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.41 mg/mL
Amount
41 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: IMR32, SK-N-AS, mouse brain, rat brain, SLIT2-transfected 293T.
Predicted reactivity: Mouse (98%), Rat (95%), Zebrafish (88%), Xenopus laevis (93%), Chicken (97%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SLIT2 are ligands for Robo receptors and play a vital role for axonal migration/navigation during neural development. A number of cleavage products through alternate splicing are reported in the literature for SLIT2 proteins. The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, and adult spinal cord.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: downregulated during adipocyte differentiation-1; leucine-rich repeat EGF-like repeat secreted protein; neurogenic extracellular slit protein; secreted ligand for robo receptors; Slit homolog 2 protein; Slit-2; unnamed protein product
Gene Aliases: Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; SLIL3; Slit-2; SLIT2
UniProt ID: (Human) O94813, (Mouse) Q9R1B9
Entrez Gene ID: (Human) 9353, (Rat) 360272, (Mouse) 20563
GTPase inhibitor activity
calcium ion binding
protein binding
heparin binding
identical protein binding
protein homodimerization activity
laminin-1 binding
proteoglycan binding
Roundabout binding
follicle-stimulating hormone receptor activity
heparan sulfate proteoglycan binding
chemorepellent activity
receptor binding
immunoglobulin superfamily cell adhesion molecule
ureteric bud development
kidney development
negative regulation of protein phosphorylation
cell migration involved in sprouting angiogenesis
negative regulation of leukocyte chemotaxis
aortic valve morphogenesis
pulmonary valve morphogenesis
chemotaxis
nervous system development
axon guidance
motor neuron axon guidance
negative regulation of lamellipodium assembly
negative regulation of endothelial cell migration
negative regulation of macromolecule metabolic process
negative regulation of neuron projection development
negative regulation of smooth muscle cell migration
olfactory bulb development
chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration
chemorepulsion involved in postnatal olfactory bulb interneuron migration
corticospinal neuron axon guidance through spinal cord
telencephalon cell migration
cell differentiation
negative regulation of cell growth
negative regulation of cell migration
negative regulation of actin filament polymerization
retinal ganglion cell axon guidance
regulation of cellular component size
cellular response to hormone stimulus
Roundabout signaling pathway
positive regulation of apoptotic process
negative regulation of vascular permeability
branching morphogenesis of an epithelial tube
axon extension involved in axon guidance
regulation of neurogenesis
regulation of axonogenesis
positive regulation of axonogenesis
negative chemotaxis
induction of negative chemotaxis
negative regulation of small GTPase mediated signal transduction
response to cortisol
ventricular septum morphogenesis
epithelial tube morphogenesis
apoptotic process involved in luteolysis
negative regulation of chemokine-mediated signaling pathway
cellular response to heparin
negative regulation of smooth muscle cell chemotaxis
negative regulation of mononuclear cell migration
circulatory system development
negative regulation of neutrophil chemotaxis
negative regulation of monocyte chemotaxis
negative regulation of retinal ganglion cell axon guidance
negative regulation of cellular response to growth factor stimulus
metanephros development
in utero embryonic development
axonogenesis
brain development
negative regulation of cell proliferation
negative regulation of gene expression
single organismal cell-cell adhesion
spinal cord development
negative regulation of axon extension
response to nutrient levels
dorsal/ventral axon guidance
follicle-stimulating hormone signaling pathway
negative regulation of catalytic activity
neuron projection morphogenesis
negative regulation of inflammatory response
mammary gland duct morphogenesis
mammary duct terminal end bud growth
multicellular organism development
negative regulation of GTPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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