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Invitrogen
SMAD3 Polyclonal Antibody
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FIGURE: 1 / 79
SMAD3 Antibody (51-1500) in ICC/IF
Immunofluorescence analysis of SMAD3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD3 Rabbit polyclonal Antibody (Product # 51-1500) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at ... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20220826?time=20220826 "SMAD3 Antibody (51-1500) in WB")
.jpg?time=20220826?time=20220826 "SMAD3 Antibody (51-1500) in WB")
.jpg?time=20220826?time=20220826 "SMAD3 Antibody (51-1500) in ChIP, EMSA")
Product Details
51-1500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Deer,
Dog,
Human,
Luciferase,
Mink,
Mouse,
Non-human primate,
Rat,
Tag,
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp586N0721; DKFZp686J10186; JV15-2; MAD homolog 3; MAD-3; MGC60396; Mothers against decapentaplegic homolog 3; SMAD; SMAD 3; SMAD family member 3
Gene Aliases: MADH3; SMAD3
Entrez Gene ID: (Human) 4088
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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