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SMAD3 Polyclonal Antibody

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SMAD3 Polyclonal Antibody

Product Details

600-401-920

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:3,000
-

ELISA (ELISA)

1:10,000-1:40,000
-
Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of human SMAD3 protein.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

SMAD3 affinity-purified antibody is directed against human Smad3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, swine, and chicken sources based on 100% homology for the immunogen sequence. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human SMAD3.

Target Information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp586N0721; DKFZp686J10186; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; TGF-beta response effector Smad3; transcription factor

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Gene Aliases: AU022421; mad3; MADH3; madh3-A; madh3a; Smad 3; SMAD3; wu:fa99e03; XELAEV_18018454mg; XenMLP; Xmad3; XSmad3

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UniProt ID: (Pig) P84024, (Chicken) P84023, (Mouse) Q8BUN5, (Rat) P84025

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Entrez Gene ID: (Pig) 397260, (Chicken) 395132, (Bovine) 515125, (Mouse) 17127, (Rat) 25631, (Xenopus) 378633, (Zebrafish) 58092

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Function(s)
core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding metal ion binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription cofactor activity transforming growth factor beta receptor binding collagen binding transcription factor binding zinc ion binding DEAD/H-box RNA helicase binding protein kinase binding phosphatase binding chromatin DNA binding ubiquitin protein ligase binding mineralocorticoid receptor binding glucocorticoid receptor binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding co-SMAD binding I-SMAD binding R-SMAD binding primary miRNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, protein binding DNA binding chromatin binding double-stranded DNA binding protein binding beta-catenin binding enzyme binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding SMAD binding protein heterodimerization activity DNA-binding transcription factor gene-specific transcriptional regulator
Process(es)
regulation of transcription, DNA-templated transforming growth factor beta receptor signaling pathway negative regulation of transcription from RNA polymerase II promoter ureteric bud development response to hypoxia mesoderm formation somitogenesis liver development heart looping osteoblast development immune system development activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest SMAD protein complex assembly endoderm development anatomical structure morphogenesis embryonic pattern specification positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway cell differentiation negative regulation of cell growth BMP signaling pathway thyroid gland development positive regulation of chondrocyte differentiation regulation of transforming growth factor beta2 production negative regulation of osteoblast proliferation nodal signaling pathway T cell activation cell-cell junction organization positive regulation of nitric oxide biosynthetic process negative regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response regulation of binding negative regulation of cytosolic calcium ion concentration pericardium development SMAD protein signal transduction negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway positive regulation of extracellular matrix assembly positive regulation of pri-miRNA transcription from RNA polymerase II promoter skeletal system development osteoblast differentiation in utero embryonic development negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter transport gastrulation negative regulation of cell proliferation positive regulation of gene expression positive regulation of alkaline phosphatase activity evasion or tolerance of host defenses by virus signal transduction involved in regulation of gene expression positive regulation of cell migration positive regulation of bone mineralization positive regulation of interleukin-1 beta production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway positive regulation of catenin import into nucleus intracellular signal transduction negative regulation of protein catabolic process positive regulation of transcription factor import into nucleus negative regulation of apoptotic process positive regulation of transcription, DNA-templated protein stabilization positive regulation of positive chemotaxis positive regulation of stress fiber assembly positive regulation of focal adhesion assembly transdifferentiation positive regulation of canonical Wnt signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway mesoderm development primitive hemopoiesis
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