Invitrogen

SMAD3 Polyclonal Antibody

1个已发表图片
2 篇参考文献
查看其他60个SMAD3抗体

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持

产品信息

PA5-17378

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:50
-

免疫沉淀 (IP)

1:25
-
产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a central region unique to Smad3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.2 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10981224

产品详细信息

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

靶标信息

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3

查看更多 收起

基因别名: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; Smad 3; SMAD3

查看更多 收起

UniProt ID: (Human) P84022, (Mouse) Q8BUN5, (Rat) P84025

查看更多 收起

Entrez Gene ID: (Human) 4088, (Mouse) 17127, (Rat) 25631

查看更多 收起

功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding transcription factor binding zinc ion binding protein kinase binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity chromatin DNA binding ubiquitin protein ligase binding enhancer binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity co-SMAD binding R-SMAD binding DNA binding chromatin binding double-stranded DNA binding beta-catenin binding enzyme binding SMAD binding metal ion binding DNA-binding transcription factor gene-specific transcriptional regulator
参与通路
negative regulation of transcription from RNA polymerase II promoter ureteric bud development response to hypoxia in utero embryonic development mesoderm formation somitogenesis liver development heart looping osteoblast development immune system development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transport activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest transforming growth factor beta receptor signaling pathway SMAD protein complex assembly endoderm development embryonic pattern specification positive regulation of gene expression positive regulation of alkaline phosphatase activity positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus signal transduction involved in regulation of gene expression negative regulation of cell growth positive regulation of cell migration positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway thyroid gland development primary miRNA processing positive regulation of chondrocyte differentiation positive regulation of interleukin-1 beta production regulation of transforming growth factor beta2 production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway negative regulation of osteoblast proliferation nodal signaling pathway wound healing T cell activation positive regulation of transcription factor import into nucleus negative regulation of apoptotic process cell-cell junction organization negative regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response positive regulation of positive chemotaxis regulation of binding positive regulation of stress fiber assembly positive regulation of focal adhesion assembly pericardium development transdifferentiation SMAD protein signal transduction negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of extracellular matrix assembly skeletal system development osteoblast differentiation negative regulation of protein phosphorylation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter gastrulation negative regulation of cell proliferation positive regulation of catenin import into nucleus intracellular signal transduction negative regulation of protein catabolic process protein stabilization positive regulation of canonical Wnt signaling pathway
guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持