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Agrisera
SMT1/Sterol methyltransferase 1 Polyclonal Antibody
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SMT1/Sterol methyltransferase 1 Antibody (AS07-266) in WB
Total protein from Col-0 (wild-type) Arabidopsis thaliana were extracted with 50 m M HEPES-KOH buffer containing 250 m M sucrose, 5% glycerol, 50 m M Na PP, 1 m M Na Mo, 25 m M Na F, 10 m M EDTA, 0.5% PVP, 3 m M DTT, 1 m M PMSF, 10 µM Leupeptin & 10 nmM Calyculin, and then fractionated by ultracentrifugation at 100,000 x gravity for 30 min at 4°C into soluble (S 100) and microsomal (P 100) proteins as descr... View More
产品信息
AS07-266
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1.
偶联物
形式
Lyophilized
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
SMT1 encodes a membrane-associated sterol C24-methyltransferase that localizes to the endoplasmic reticulum as part of the sterol biosynthetic machinery, positioning its catalytic activity at the site of bulk lipid synthesis. The gene produces a single polypeptide enzyme with the conserved S-adenosylmethionine-dependent sterol methyltransferase fold typical of ERG6-like proteins, functioning as an integral component of the pathway that alkylates the sterol side chain at carbon 24. Biochemically, SMT1 catalyzes methyl transfer reactions that convert Delta(24)-sterol intermediates toward C24-alkylated plant sterols, thereby constraining cholesterol accumulation and supporting proper membrane composition. Genetic and functional studies in Arabidopsis show that loss of SMT1 disrupts sterol profiles (with increased cholesterol and reduced C24-alkylated sterols) and causes pleiotropic developmental defects, including impaired growth, fertility, root calcium sensitivity, and abnormal embryo morphogenesis, indicating that SMT1-dependent sterol products are required for normal development beyond brassinosteroid-related outputs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G13710; CEPHALOPOD; CPH; MSH12.18; MSH12_18; SAM:CYCLOARTENOL-C24-METHYLTRANSFERASE
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