Invitrogen

SOX11 Polyclonal Antibody

查看其他25个SOX11抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)

SOX11 Antibody in Immunocytochemistry (ICC/IF)

SOX11 Antibody in Immunocytochemistry (ICC/IF)

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SOX11 Antibody (PA5-115950) in ICC/IF

Immunocytochemistry analysis of SOX11 in 3T3 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115950) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

SOX11 Antibody in Immunocytochemistry (ICC/IF)

SOX11 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SOX11 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SOX11 in 3T3 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115950) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

SOX11 Antibody in Western Blot (WB) — Western blot analysis of SOX11 in HeLa cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115950).

Immunogen image

SOX11 Polyclonal Antibody

Immunogen Image SOX11 Polyclonal Antibody

产品信息

PA5-115950

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SOX11(Accession P35716), corresponding to amino acid residues E378-D428.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total SOX11.

靶标信息

This intronless gene encodes a member of the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: SRY; SRY-box 11; SRY-box containing gene 11; Transcription factor SOX-11

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基因别名: 1110038H03Rik; 6230403H02Rik; AI836553; end1; Sox-11; SOX11

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UniProt ID: (Mouse) Q7M6Y2, (Rat) P0C1G9

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Entrez Gene ID: (Mouse) 20666, (Rat) 84046

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding translation factor activity, RNA binding protein binding HMG box transcription factor

negative regulation of transcription from RNA polymerase II promoter skeletal system development neural tube formation lens morphogenesis in camera-type eye outflow tract morphogenesis cardiac ventricle formation noradrenergic neuron differentiation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter translation multicellular organism development nervous system development positive regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression glial cell proliferation neural crest cell development spinal cord development glial cell development cell differentiation positive regulation of BMP signaling pathway positive regulation of hippo signaling skeletal muscle cell differentiation positive regulation of neuron differentiation positive regulation of osteoblast differentiation positive regulation of ossification positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion sympathetic nervous system development embryonic digestive tract morphogenesis embryonic skeletal system morphogenesis negative regulation of lymphocyte proliferation positive regulation of neurogenesis hard palate development soft palate development limb bud formation negative regulation of glial cell proliferation ventricular septum morphogenesis lung morphogenesis negative regulation of cell death neuroepithelial cell differentiation eyelid development in camera-type eye somite development cornea development in camera-type eye closure of optic fissure positive regulation of stem cell proliferation negative regulation of transcription regulatory region DNA binding positive regulation of lens epithelial cell proliferation kidney development cell proliferation oligodendrocyte development regulation of transforming growth factor beta receptor signaling pathway neuron differentiation signal transduction involved in cell cycle checkpoint