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Invitrogen
SP1 Polyclonal Antibody
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SP1 Antibody (PA5-95583) in ICC/IF
Immunofluorescence analysis of SP1 was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SP1 Polyclonal Antibody (Product # PA5-95583) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2... View More
产品信息
PA5-95583
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat,
Human,
Mouse
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human SP1 recombinant protein (Position: Q384-A603).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human A431 whole cell, human 293T whole cell, human Hela whole cell. IHC: human non-samll cell lung carcinoma tissue, human ovarian cancer tissue, mouse lung tissue, rat lung tissue. ICC/IF: A549 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HGNC:11205; Specificity protein 1; trans-acting transcription factor 1; Transcription factor Sp1
基因别名: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1
UniProt ID: (Human) P08047, (Mouse) O89090, (Rat) Q01714
Entrez Gene ID: (Human) 6667, (Mouse) 20683, (Rat) 24790
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
RNA polymerase II repressing transcription factor binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein C-terminus binding
transcription factor binding
histone acetyltransferase binding
protein homodimerization activity
histone deacetylase binding
bHLH transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
HMG box domain binding
RNA polymerase II core promoter sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
enhancer binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
C2H2 zinc finger transcription factor
regulation of transcription, DNA-templated
viral process
snRNA transcription from RNA polymerase II promoter
positive regulation by host of viral transcription
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of phenotypic switching by transcription from RNA polymerase II promoter
positive regulation of hydrogen sulfide biosynthetic process
ossification
in utero embryonic development
trophectodermal cell differentiation
liver development
embryonic placenta development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
megakaryocyte differentiation
lung development
enucleate erythrocyte differentiation
embryonic camera-type eye morphogenesis
embryonic skeletal system development
embryonic process involved in female pregnancy
definitive hemopoiesis
