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Invitrogen
SPARC Monoclonal Antibody (ON1-1)
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SPARC Antibody (33-5500) in ICC/IF
Asian journal of andrology 2020 -
Figure 2 Basal expression, silencing, and overexpression of SPARC in PCa cell lines. (a) Representative image of western blot against SPARC in PCa cell lines 22Rv1, LNCaP, DU145 and PC3. (b) RT-qPCR of SPARC in PCa cell lines. DeltaDeltaCt was obtained after normalizing to PUM1 and PC3 cell lines ( n = 3). (c and d) PC3 cells were stable transduced with shRNA against SPARC (ShSPARC) or scramble (ShScr). LNC... View More
产品信息
33-5500
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Pig,
Rabbit
已发表种属
Dog,
Human,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
ON1-1
抗原
Full-length Bovine bone osteonectin (SPARC)
偶联物
形式
Lyophilized
浓度
2.0 mg/mL
纯化类型
Ion-exchange chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with bovine bone osteonectin (SPARC). The monoclonal antibody was harvested from ascitic fluid.
Specificity: This antibody reacts with both bone derived and platelet derived Osteonectin (ELISA). This antibody does not react with non-activated platelet and reacts with thrombin-stimulated platelet (flow cytometric analysis). This antibody reacts with human and bovine Osteonectin (Western blot analysis). This antibody cross reacts with rabbit and porcine Osteonectin. (ELISA)
Storage: 4°C. This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
靶标信息
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; osteonectin precursor; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC
基因别名: BM-40; OI17; ON; SPARC
UniProt ID: (Bovine) P13213, (Human) P09486, (Pig) P20112
Entrez Gene ID: (Bovine) 282077, (Human) 6678, (Pig) 595124, (Rabbit) 100009355
ossification
negative regulation of endothelial cell proliferation
platelet degranulation
receptor-mediated endocytosis
signal transduction
heart development
response to gravity
response to lead ion
positive regulation of endothelial cell migration
negative regulation of angiogenesis
regulation of cell morphogenesis
extracellular matrix organization
lung development
response to lipopolysaccharide
response to L-ascorbic acid
response to cytokine
wound healing
response to peptide hormone
response to ethanol
response to cadmium ion
inner ear development
response to glucocorticoid
response to cAMP
response to calcium ion
bone development
cellular response to growth factor stimulus
