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Bethyl Laboratories
SPF30 Polyclonal Antibody
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FIGURE: 1 / 1
SPF30 Antibody (A302-836A) in WB
Detection of human and mouse SPF30 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SPF30 antibody (Product # A302-836A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SPF30 antibody A302-837A, which recognizes a downstream epitope. Detection: Chemiluminescence with e... View More
Product Details
A302-836A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of Human Splicing Factor 30.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Orangutan
Target Information
This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 30 kDa splicing factor SMNrp; MGC106917; MGC112663; SF; SMN related protein; SMN-related protein; splicing factor 30, survival of motor neuron-related; Survival motor neuron domain-containing protein 1; Survival of motor neuron-related-splicing factor 30; tudor domain containing 16C; unnamed protein product
Gene Aliases: 2410004J23Rik; 4933440I19Rik; SMNDC1; SMNR; SPF30; TDRD16C
UniProt ID: (Human) O75940, (Mouse) Q8BGT7
Entrez Gene ID: (Human) 10285, (Mouse) 76479
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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