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SPSC/Sucrose phosphate synthase isoform C Polyclonal Antibody
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SPSC/Sucrose phosphate synthase isoform C Antibody (AS15-2997) in WB
Proteins were isolated from Arabidopsis thaliana wild-type and SPS mutants were solubilized with 3 X LB (6 M urea, 12% SDS, 30% glycerol, 100 m M DTT, 150 m M Tris p H 7.0,0.8% Comassie G-250). 10 µg of proteins were loaded into each lane and separated on 10% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody so... View More
产品信息
AS15-2997
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPS4 sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Phaseolus vulgaris
靶标信息
SPSC encodes an Arabidopsis thaliana sucrose-phosphate synthase family enzyme that is predominantly cytosolic and functions in primary carbon metabolism by catalyzing sucrose-6-phosphate formation from UDP-glucose and fructose-6-phosphate, a key regulatory step of sucrose biosynthesis that links photosynthetic carbon export in the light and starch-to-sucrose conversion at night; consistent with this role, Arabidopsis SPSC is one of four partially redundant SPS isoforms whose combined activity is important for normal growth, respiration, and carbon partitioning. The SPSC protein is a large, soluble glycosyltransferase of the GT4 superfamily, built around a conserved N-terminal catalytic glucosyltransferase region with characteristic nucleotide-sugar binding features and an extended C-terminal region found in plant SPS proteins that contributes to regulation and functional diversification, with multiple serine residues reported as phosphorylation sites, supporting regulation by reversible phosphorylation in response to cellular and environmental cues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G10120; ATSPS4F; AtSPSC; F28M11.40; SPS4; SPS4F
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