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Invitrogen
SQSTM1 Polyclonal Antibody
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图: 1 / 8
SQSTM1 Antibody (PA5-95255) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of SQSTM1 in A431 cell using SQSTM1 Polyclonal Antibody (Product # PA5-95255). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter se... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95255
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62 (69-96aa DEDGDLVAFSSDEELTMAMSYVKDDIFR).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human Jurkat whole cell, rat liver tissue, rat brain tissue, mouse liver tissue. IHC: mouse small intestine tissue, human intestinal cancer tissue, rat small intestine tissue IHC-F: human placenta tissue. ICC/IF: A431 cell, A549 Cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: oxidative stress induced; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62
基因别名: A170; OSF-6; Osi; p62; Sqstm1; STAP; STONE14; ZIP; ZIP3
UniProt ID: (Rat) O08623, (Mouse) Q64337
Entrez Gene ID: (Rat) 113894, (Mouse) 18412
positive regulation of protein phosphorylation
immune system process
ubiquitin-dependent protein catabolic process
autophagy
apoptotic process
macroautophagy
positive regulation of macroautophagy
cell differentiation
regulation of I-kappaB kinase/NF-kappaB signaling
protein heterooligomerization
regulation of mitophagy
mitophagy in response to mitochondrial depolarization
regulation of nucleic acid-templated transcription
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
