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Agrisera
SRK2E/Ser/Thr-protein kinase SnRK2,6 Polyclonal Antibody
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SRK2E/Ser/Thr-protein kinase SnRK2,6 Antibody (AS13-2635) in WB
Bacterial lysates were separated on 12% SDS-PAGE and blotted 1 h to PVDF using semi-dry or tank transfer. Blots were blocked with 5% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:3,000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min i... View More
产品信息
AS13-2635
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated unique synthetic peptide derived from Arabidopsis thaliana SRK2E sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
SRK2E encodes a plant-specific SNF1-related protein kinase 2 that functions in Arabidopsis stress and hormone signaling; it is a soluble, calcium-independent serine/threonine kinase that primarily operates in the nucleus and cytoplasm as part of abscisic acid (ABA)-responsive pathways. The protein is organized around an N-terminal catalytic kinase domain typical of eukaryotic protein kinases, followed by a C-terminal regulatory region required for stimulus-dependent activation; functional dissection of this C-terminus indicates separable modules that differentially support ABA-dependent versus ABA-independent (osmotic stress) activation, and the ABA-specific portion mediates interaction with the PP2C phosphatase ABI1. Biologically, SRK2E acts as a positive regulator of ABA-induced stomatal closure and broader drought/osmotic stress responses: genetic disruption impairs ABA control of stomatal aperture, while SnRK2 pathway disruption compromises drought tolerance and ABA-responsive gene regulation, consistent with SRK2E functioning upstream of stress-responsive transcriptional programs and water-conservation physiology.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G33950; ATOST1; F17I5.140; F17I5_140; OPEN STOMATA 1; OST1; P44; SNF1-RELATED PROTEIN KINASE 2.6; SnRK2-6; SnRK2.6; SUCROSE NONFERMENTING 1-RELATED PROTEIN KINASE 2-6
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