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Agrisera
SRP43/Signal recognition peptide 43 Polyclonal Antibody
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SRP43/Signal recognition peptide 43 Antibody (AS12-2105) in WB
15 µg/well of total protein extracted freshly from rosetta leaves of Arabidopsis thaliana with 2 X LaemmLi buffer and denatured at 95°C/5 min. Samples were separated in the cold on 12% SDS-PAGE and blotted for 1.5 h to nitrocellulose (pore size of 0.45 µm), using: wet, semi-dry transfer in the cold. Blot was blocked with 5% milk for: 1 h/RT with agitation. Blot was incubated in the primary antibody at a dil... View More
产品信息
AS12-2105
产品规格
种属反应
Plant
宿主/亚型
Chicken
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
SRP43 encodes a chloroplast-localized, soluble signal recognition particle subunit that accumulates in the stroma and functions at the thylakoid membrane during post-translational targeting. The protein is a plant-specific multidomain factor of ~43 kDa built from a central ankyrin-repeat region and an N-terminal chromodomain module (three chromodomains have been described in canonical cpSRP43 proteins), creating multiple linear-motif binding surfaces for partner recognition. Functionally, SRP43 is a core component of the chloroplast SRP pathway that forms a soluble transit complex with cpSRP54 and directly binds light-harvesting chlorophyll a/b-binding proteins (LHCPs) to prevent their aggregation after import into the chloroplast; the ankyrin repeats mediate high-affinity recognition of an LHCP motif and are sufficient for the ATP-independent chaperone activity, while chromodomain-dependent interactions contribute to docking and coordination with other pathway components such as the Alb3 insertase and cpSRP54 to promote efficient delivery and insertion of LHCPs into the thylakoid membrane.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: At2G47450; CAO; CPSRP43; T30B22.25
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