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Invitrogen
SRSF3 Polyclonal Antibody
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SRSF3 Antibody (PA5-80070) in ICC/IF
Immunocytochemistry analysis of SRSF3 in A549 cells. Samples were incubated with SRSF3 polyclonal antibody (Product # PA5-80070) using a dilution of 5 μg/mL overnight at 4°C followed by DyLight®488 Conjugated Goat Anti-Rabbit IgG and DyLight®550 Conjugated Goat Anti-Mouse IgG for 30 minutes at 37°C. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples incubated with primary and mouse anti-Beta Tubulin antibody. Visualize using a fluorescence micro... View More
产品信息
PA5-80070
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human SFRS3 (1-29aa MHRDSCPLDCKVYVGNLGNNGNKTELERA).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SH-SY5Y whole cell, human 293T whole cell, human HepG2 whole cell, human SiHa whole cell. ICC/IF: A549 cell. Flow: SH-SY5Y cells.
靶标信息
SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD
基因别名: SFRS3; SRP20; SRSF3
UniProt ID: (Human) P84103
Entrez Gene ID: (Human) 6428
