Invitrogen

SSB Polyclonal Antibody

Name: SSB Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29763

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2 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

SSB Antibody in Immunocytochemistry (ICC/IF)

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SSB Antibody (PA5-29763) in ICC/IF

Immunocytochemistry analysis of SSB in paraformaldehyde-fixed A431. Samples were incubated with SSB polyclonal antibody (Product # PA5-29763) using a dilution of 1:200.

SSB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

SSB Antibody — Subcellular localization of La/Sjogren's syndrome-related antigen B (SSB) antigen under normal conditions and apoptosis in vitro , as shown by immunofluorescence staining of human salivary gland (HSG) cells. La/SSB (SSB) was stained with polyclonal antibody followed by AlexaFluor-488 secondary antibody (green) and nuclear DNA was stained with propidium iodide (PI) (red). In (a), under normal conditions La/SSB is increased in the cytoplasm showing a diffuse staining pattern and in a lower level in the nucleus. In (c) a merged image of (a) and (b) indicates that La/SSB is partially intermingled with PI-positive DNA (yellow, indicating overlapping green and red pixels) in untreated cells. Following treatment with 10 muM thapsigargin for 24 h La/SSB showed a striking pattern of relocalization to the periphery, forming thin eccentric rims or crescents inside the cell membrane (d,f). At later stages of apoptosis, La/SSB was found to be exposed on the surface of apoptotic cells, present in blebs budding from the membrane and on apoptotic bodies (g,i). {TM}

产品信息

PA5-29763

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human SSB. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.52 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2547237

产品详细信息

Recommended positive controls: 293T, JC, C2C12.

Predicted reactivity: Mouse (89%), Rat (89%), Chimpanzee (99%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: autoantigen La; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family, member 3; lupus La antigen; Lupus La protein; Lupus La protein homolog; Lupus LA protein homolog (LA ribonucleoprotein) (LA autoantigen homolog); mRNA for autoantigen; OTTHUMP00000204777; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B (autoantigen La); SS-B; SS-B/La protein

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基因别名: La; La/SSB; LARP3; SS-B; SSB

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UniProt ID: (Human) P05455, (Mouse) P32067

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Entrez Gene ID: (Human) 6741, (Rat) 81783, (Mouse) 20823

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功能

tRNA binding nucleotide binding mRNA binding protein binding poly(A) RNA binding RNA binding nucleic acid binding RNA metabolism protein

参与通路

tRNA modification histone mRNA metabolic process IRES-dependent viral translational initiation protein localization to cytoplasmic stress granule RNA processing tRNA processing