Invitrogen

STIM1 Polyclonal Antibody

Name: STIM1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-96668

查看其他27个STIM1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)

STIM1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

STIM1 Antibody (PA5-96668) in ICC/IF

Immunofluorescence analysis of STIM1 in NIH/3T3 cells. Samples were incubated with STIM1 Polyclonal antibody (Product # PA5-96668) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

STIM1 Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image STIM1 Polyclonal Antibody

产品信息

PA5-96668

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 530-685 of human STIM1 (NP_0031472)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.75 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2808470

产品详细信息

Immunogen sequence: RQRVAPKPPQ MSRAADEALN AMTSNGSHRL IEGVHPGSLV EKLPDSPALA KKALLALNHG LDKAHSLMEL SPSAPPGGSP HLDSSRSHSP SSPDPDTPSP VGDSRALQAS RNTRIPHLAG KKAVAEEDNG SIGEETDSSP GRKKFPLKIF KKPLKK; Positive Samples: LO2, Jurkat, BxPC-3, HeLa, Mouse brain, Mouse heart, Mouse kidney; Cellular Location: Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Single-pass type I membrane protein, cytoskeleton

靶标信息

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

仅用于科研。不用于诊断过程。未经明确授权不得转售。