Invitrogen

STUB1 Monoclonal Antibody (13I8)

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说明书
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说明书
实验方案
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STUB1 Monoclonal Antibody (13I8)

产品信息

MA5-49189

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL
-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL
-

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells
-
产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

13I8

抗原

E.coli-derived human STUB1 recombinant protein (Position: R51-Y303).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_3074847

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human HEK293 whole cell, monkey COS-7 whole cell, human CACO-2 whole cellhuman THP-1 whole cell, human PC-3 whole cell, human U87 whole cell, rat heart tissue, rat brain tissue, mouse brain tissue, mouse HEPA1-6 whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human mammary cancer tissue, mouse small intestine tissue, rat small intestine tissue. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; heat shock protein A binding protein 2 (c-terminal); LA16c-313D11.6; RING-type E3 ubiquitin transferase CHIP; serologically defined colon cancer antigen 7; STIP1 homology and U box-containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase

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基因别名: 0610033N24Rik; 2210017D18Rik; 2310040B03Rik; AW046544; CHIP; HSPABP2; NY-CO-7; PP1131; SCAR16; SDCCAG7; STUB1; UBOX1

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UniProt ID: (Human) Q9UNE7, (Mouse) Q9WUD1, (Rat) A6HD62

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Entrez Gene ID: (Human) 10273, (Mouse) 56424, (Rat) 287155

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功能
G-protein coupled receptor binding ubiquitin-protein transferase activity protein binding ligase activity enzyme binding kinase binding Hsp70 protein binding protein binding, bridging TPR domain binding ubiquitin protein ligase binding ubiquitin-ubiquitin ligase activity protein homodimerization activity SMAD binding misfolded protein binding Hsp90 protein binding ubiquitin protein ligase activity ubiquitin protein ligase activity involved in ERAD pathway UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity heat shock protein binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase
参与通路
protein polyubiquitination DNA repair ubiquitin-dependent protein catabolic process misfolded or incompletely synthesized protein catabolic process protein ubiquitination negative regulation of transforming growth factor beta receptor signaling pathway ubiquitin-dependent SMAD protein catabolic process endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination regulation of protein stability regulation of glucocorticoid metabolic process negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process ERAD pathway ERBB2 signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-protein transferase activity protein maturation protein autoubiquitination protein K63-linked ubiquitination cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly protein folding cellular response to DNA damage stimulus
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