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Agrisera
SYP121/Syntaxin 121 Polyclonal Antibody
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FIGURE: 1 / 1
SYP121/Syntaxin 121 Antibody (AS22-4827) in WB
Samples: Marker: Precision Plus Dual Color Standard (Bio-Rad) 1-20 µg of Arabidopsis thaliana total protein extract 2-20 µg of Arabidopsis thaliana cytosolic fraction 3-20 µg of Arabidopsis thaliana microsomal fraction 4-20 µg of Arabidopsis thaliana plasma membrane fraction 5-20 µg of Arabidopsis thaliana other membranes fraction Arabidopsis thaliana plants were grown for 13 days at 22°C with a time regime of 16 hday/8 h night. Plants were grown on 1/2 MS agar plates, p H=5.8. Whole plants were used for the experiment. Samples were extrac... View More
Product Details
AS22-4827
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana SYP121 protein sequence.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
SYP121 encodes a plasma membrane-resident Qa-SNARE (syntaxin) that concentrates at the cell surface where secretory vesicles fuse and, in highly polarized contexts, is targeted to discrete plasma membrane domains (e.g., guard-cell and tip-growing cell regions). The protein is a single-pass membrane anchor with a C-terminal transmembrane helix, preceded by a cytosolic SNARE (Qa) motif that forms coiled-coil complexes with partner SNAREs, and an N-terminal regulatory region that includes an Habc-like folded domain and short N-terminal motifs important for partner binding and control of SNARE assembly. Functionally, SYP121 promotes post-Golgi exocytosis to the plasma membrane and helps define cargo delivery routes, thereby supporting growth and cell surface remodeling; it also couples membrane trafficking to transport activity by directly regulating and/or recycling key plasma membrane channels, including inward-rectifying K+ channels that drive guard-cell osmotic uptake during stomatal opening, and aquaporins that tune membrane water permeability. Beyond housekeeping secretion, SYP121 contributes to stimulus-responsive membrane fusion through regulated SNARE complex formation (including phosphorylation-dependent enhancement of pairing with R-SNARE partners) and participates in processes such as stomatal dynamics, polarized cell expansion, and defense-related cell wall fortification at attempted pathogen entry sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT-SYR1; ATSYP121; ATSYR1; F26K24.11; PEN1; PENETRATION1; SYNTAXIN RELATED PROTEIN 1; SYR1
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