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Stella Antibody (711460) in ICC/IF
For immunofluorescence analysis, NCCIT cells were fixed and permeabilized for detection of endogenous DPPa3 using DPPa3 Recombinant Rabbit Superclonal™ Antibody (Product # 711460, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DPPa3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938).... View More
产品信息
711460
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
17HCLC
抗原
Protein corresponding to Human DPPA3 (aa 1-159)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Rat and Horse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
DPPa3 (stella-related protein) is a stem cell marker protein which is restricted to pluripotent human embryonic stem cells, premeiotic germ lineage cells and testicular germ cell tumor cells. Otherwise known as 'developmental pluripotency-associated protein 3,' it is expressed in primordial germ cells, Oocytes, preimplantation embryos and pluripotent cells. This protein has a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Developmental pluripotency-associated protein 3; Stella-related protein
基因别名: DPPA3; STELLA; STELLAR
UniProt ID: (Human) Q6W0C5
Entrez Gene ID: (Human) 359787
