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Invitrogen
Survivin Monoclonal Antibody (J.33.5)
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Survivin Antibody (MA5-15077) in ICC/IF
Immunofluorescence analysis of Survivin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Survivin Monoclonal Antibody (J.33.5) (Product # MA5-15077) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More
产品信息
MA5-15077
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
J.33.5
抗原
Synthetic peptide corresponding to residues surrounding cysteine 60 of human Survivin
偶联物
形式
Liquid
浓度
311 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; survivin; survivin variant 3 alpha; SVV; TIAP
基因别名: AAC-11; AP14; API4; BIRC5; EPR-1; IAP4; survivin40; TIAP
UniProt ID: (Human) O15392, (Rat) Q9JHY7, (Mouse) O70201
Entrez Gene ID: (Human) 332, (Rat) 64041, (Mouse) 11799
ubiquitin-protein transferase activity
cysteine-type endopeptidase inhibitor activity
protein binding
microtubule binding
Ran GTPase binding
enzyme binding
identical protein binding
metal ion binding
chaperone binding
molecular_function
zinc ion binding
tubulin binding
protein homodimerization activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
protein heterodimerization activity
cofactor binding
peptidase inhibitor activity
G2/M transition of mitotic cell cycle
microtubule cytoskeleton organization
transcription, DNA-templated
protein phosphorylation
chromosome segregation
regulation of mitotic cell cycle
regulation of signal transduction
protein ubiquitination
protein complex localization
positive regulation of exit from mitosis
cellular response to stem cell factor stimulus
response to drug
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to peptide hormone
negative regulation of neuron apoptotic process
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
cell division
establishment of chromosome localization
response to glucocorticoid
regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of type B pancreatic cell proliferation
cellular response to ethanol
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
apoptotic process
sister chromatid cohesion
positive regulation of cell proliferation
protein sumoylation
regulation of apoptotic process
cytokinesis
regulation of transcription, DNA-templated
cell cycle
mitotic nuclear division
embryo development
negative regulation of peptidase activity
spindle checkpoint
