Invitrogen

Synaptophysin Monoclonal Antibody (SP11)

Name: Synaptophysin Monoclonal Antibody (SP11)
Brand: Invitrogen
SKU: MA5-16402

高级验证

7个已发表图片

42 篇参考文献

查看其他44个Synaptophysin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Synaptophysin Antibody in Immunocytochemistry (ICC/IF)

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Synaptophysin Antibody (MA5-16402) in ICC/IF

Immunofluorescence analysis of Synaptophysin was performed using neuronal differentiated SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Synaptophysin Monoclonal Antibody (SP11) (Product # MA1-39558, MA5-16402) at 1:200 dilution in 0.1% BSA, i... View More

Synaptophysin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Synaptophysin Antibody in Western Blot (WB)

Synaptophysin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Synaptophysin Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image Synaptophysin Monoclonal Antibody (SP11)

产品信息

MA5-16402

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看28篇已发表文章 28篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

1:200

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

SP11

抗原

Synthetic peptide corresponding to C-terminus of human synaptophysin.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant/TBS, pH 7.5, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

AB_2537921

产品详细信息

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is pancreas or neuroendocrine tumor.

靶标信息

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

仅用于科研。不用于诊断过程。未经明确授权不得转售。