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Invitrogen
Synaptotagmin 1 Polyclonal Antibody
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FIGURE: 1 / 8
Synaptotagmin 1 Antibody (PA5-80089) in IHC (P)
Immunohistochemistry analysis of Synaptotagmin 1 on paraffin-embedded human melanoma tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Synaptotagmin 1 polyclonal antibody (Product# PA5-80089) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 min... View More
Product Details
PA5-80089
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human Synaptotagmin 1 (RRPIAQWHTLQVEEEVDAMLAVKK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue. IHC: human glioma tissue, human melanoma tissue, mouse brain tissue, rat brain tissue. Flow: A549 cell, PC-3 cell, LLC cell.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin XI; Synaptotagmin-1; Synaptotagmin-2; SYT2; SytI; SytII
Gene Aliases: AW124717; G630098F17Rik; P65; SVP65; SYT; SYT1; SytI
UniProt ID: (Human) P21579, (Rat) P21707, (Mouse) P46096
Entrez Gene ID: (Human) 6857, (Rat) 25716, (Mouse) 20979
SNARE binding
phosphatidylserine binding
calcium ion binding
protein binding
calmodulin binding
calcium-dependent phospholipid binding
1-phosphatidylinositol binding
phosphatidylinositol-4,5-bisphosphate binding
protein C-terminus binding
syntaxin-1 binding
clathrin binding
identical protein binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
transporter activity
phospholipid binding
syntaxin binding
metal ion binding
membrane trafficking regulatory protein
detection of calcium ion
vesicle fusion
chemical synaptic transmission
neurotransmitter secretion
brain development
glutamate secretion
regulation of exocytosis
regulation of calcium ion-dependent exocytosis
positive regulation of vesicle fusion
positive regulation of calcium ion-dependent exocytosis
vesicle docking
synaptic vesicle endocytosis
calcium ion-regulated exocytosis of neurotransmitter
positive regulation of synaptic transmission
protein homooligomerization
regulation of synaptic transmission, glutamatergic
cellular response to calcium ion
fast, calcium ion-dependent exocytosis of neurotransmitter
regulation of regulated secretory pathway
positive regulation of dendrite extension
synaptic vesicle exocytosis
calcium ion regulated exocytosis
response to calcium ion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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