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Rockland
T4 RNA Ligase Polyclonal Antibody
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T4 RNA Ligase Antibody (200-401-MX4) in WB
Western Blot Results of Rabbit Anti-T4 RNA Ligase Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB210-0500) [10 µL]. Lane 2: T4 RNA Ligase [0.5 µg]. Lane 3: T7 RNA Polymerase [0.5 µg]. Lane 4: Bovine Albumin (BSA) (p/n 001-0133) [0.5 µg]. Primary Antibody: Rabbit Anti-Anti-T4 RNA Ligase Antibody at 1: 20,000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase Conjugated (p/n 611-1302) at 1: 40,000 at RT for 30 mins. Block: BlockOut Universal Blocking Buffer (p/n MB-073) at RT for 1 hr. Block: BlockOut U... View More
产品信息
200-401-MX4
产品规格
种属反应
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-T4 RNA Ligase antibody was produced from whole rabbit serum produced by repeated immunizations with T4 Ligase isolated from E. coli.
偶联物
形式
Liquid
浓度
1.19 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
T4 RNA ligase is an enzyme derived from bacteriophage T4, which is primarily involved in the catalysis of RNA molecules. This ligase facilitates the joining of single-stranded RNA or DNA by forming phosphodiester bonds between 3'-hydroxyl and 5'-phosphate termini. Structurally, T4 RNA ligase is composed of distinct domains that contribute to its function as a ligase, including a catalytic domain essential for the reaction. It is frequently employed in molecular biology laboratories for constructing cDNA libraries, RNA sequencing, and cloning applications due to its ability to ligate oligonucleotides, ribonucleotides, and adaptors efficiently. T4 RNA ligase has also been instrumental in site-specific labeling and the study of RNA structure and function, including splicing mechanisms and RNA repair processes. Its versatility as a biotechnological tool is underscored by its high efficiency and specificity, making it a critical component in RNA research and applications. Advances in understanding the enzyme's mechanism have facilitated the development of improved ligation methods, enhancing various RNA technologies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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