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TBK1 Antibody (PA5-17478) in ICC/IF
Immunofluorescence analysis of TBK1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TBK1 Polyclonal Antibody (Product # PA5-17478) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # PA5-17478) at... View More
产品信息
PA5-17478
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues near the carboxy-terminus of TBK1/NAK
偶联物
形式
Liquid
浓度
174 µg/mL
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
TBK1 serine/threonine kinase is a member of the IKK family and is involved in NF-kappa B activation and IRF3 signaling. TBK1 is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKK beta, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKK beta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk
基因别名: 1200008B05Rik; AI462036; AW048562; FTDALS4; NAK; T2K; TBK1
UniProt ID: (Human) Q9UHD2, (Mouse) Q9WUN2
Entrez Gene ID: (Human) 29110, (Mouse) 56480, (Rat) 299827
nucleic acid binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
phosphoprotein binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
inflammatory response
I-kappaB kinase/NF-kappaB signaling
response to virus
negative regulation of gene expression
peptidyl-serine phosphorylation
negative regulation of type I interferon production
positive regulation of type I interferon production
type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of peptidyl-serine phosphorylation
TRIF-dependent toll-like receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
dendritic cell proliferation
innate immune response
positive regulation of interferon-beta biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-positive bacterium
defense response to virus
regulation of neuron death
positive regulation of xenophagy
activation of innate immune response
immune system process
protein phosphorylation
phosphorylation
