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Invitrogen
TCR H-Y (male antigen) Monoclonal Antibody (T3.70), FITC, eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "TCR H-Y (male antigen) Antibody in Flow Cytometry (Flow)")
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TCR H-Y (male antigen) Antibody (11-9930-82) in Flow
The CD11.3 cell line was stained with 0.125 µg of Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or 0.125 µg of Anti-Mouse H-Y TCR (male antigen) FITC (purple histogram). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "TCR H-Y (male antigen) Antibody (11-9930-82) in Flow")
产品信息
11-9930-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
T3.70
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The T3.70 monoclonal antibody reacts with the transgenic alpha betaTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4^+ and CD8^+ T cell differentiation.
Applications Reported: The T3.70 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4+ and CD8+ T cell differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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