Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TCR V delta 1 Monoclonal Antibody (TS8.2), Brilliant Violet™ 421, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 1
TCR V delta 1 Antibody (404-5679-42) in Flow
Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0038-42), TCR gamma/delta Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-9959-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet 421 (Product # 404-4714-81) (left) or TCR V delta 1 Monoclonal Antibody, Brilliant Violet 421 (right). Viable CD3 positive cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
404-5679-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
TS8.2
抗原
Full length protein of Human TCR delta v1
偶联物
激发/发射光谱
406/423 nm
查看光谱
形式
liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Description
This TS8.2 monoclonal antibody recognizes human TCR V delta 1, a common framework determinant on the delta-subunit of the T cell receptor. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity. TCR V delta 1 Monoclonal Antibody (TS8.2) has been validated for the following applications Flow Cytometry
Applications Tested
This TS8.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
靶标信息
The T Cell Receptor gamma-delta (TCR gamma delta) is a heterodimeric membrane receptor that consists of the highly variable gamma and delta chains expressed as part of a complex with the invariant CD3 proteins. Gamma-delta T cells are less frequent than alpha-beta T cells but compose a substantial part of T cells found at epithelial borders including the gut mucosa resident intraepithelial lymphocytes (IEL). The antigenic stimuli required for gamma-delta T cell receptor activation are not well understood and are thought to be mainly MHC class-independent.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
