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Invitrogen
TEB4 Polyclonal Antibody
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TEB4 Antibody (PA5-103816) in ICC/IF
Immunocytochemistry analysis of TEB4 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TEB4 polyclonal antibody (Product # PA5-103816) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103816
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MARCH6(Accession O60337), corresponding to amino acid residues Q866-E910.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total MARCH6.
靶标信息
MARCH6 is an E3 ubiquitin ligase that localizes to endoplasmic reticulum membranes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 6-Mar; Doa10 homolog; E3 ubiquitin-protein ligase MARCH6; E3 ubiquitin-protein ligase MARCHF6; MARCH-VI; membrane associated ring finger 6; membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 6; membrane-associated RING-CH finger protein 6; Membrane-associated RING-CH protein VI; Protein TEB-4; RING finger protein 176; RING-type E3 ubiquitin transferase MARCHF6
基因别名: 3830408G03; DOA10; F830029L24Rik; KIAA0597; MARCH-VI; MARCH6; MARCHF6; mKIAA0597; RNF176; TEB4
UniProt ID: (Human) O60337, (Mouse) Q8BJA0
Entrez Gene ID: (Human) 10299, (Mouse) 223455
ubiquitin-protein transferase activity
zinc ion binding
ligase activity
enzyme binding
ubiquitin conjugating enzyme binding
ubiquitin protein ligase activity involved in ERAD pathway
ubiquitin-specific protease binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
